Inoculated culture plates were incubated for 24 hours. Negative gelatin hydrolysis exhibited by Escherichia coli (B) is indicated by the absence of a clearing zone around the colony. Positive gelatin hydrolysis exhibited by Bacillus subtilis (A) is indicated by the clear zone around the colony after the addition of saturated ammonium sulfate. Gelatin hydrolysis test using the nutrient gelatin plate method. Torres, University of Santo Tomas, Manila, Philippines).įigure 3: Gelatin hydrolysis test. (Thomas Edison dela Cruz and Jeremy Martin O. After 30 minutes in an ice bath, the uninoculated control remained solid. Torres, University of Santo Tomas, Manila, Philippines).įigure 2: Gelatin hydrolysis test. Gelatin hydrolysis was observed after 3 days of incubation. After 30 minutes in an ice bath, the nutrient gelatin tube inoculated with Bacillus subtilis exhibited positive gelatin hydrolysis as shown by medium liquefaction.
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